Journal: International Journal of Molecular Sciences

Article Title: IPS (In-Plant System) Delivery of Double-Stranded Vitellogenin and Vitellogenin receptor via Hydroponics for Pest Control in Diaphorina citri Kuwayama (Hemiptera: Psyllidae)

doi: 10.3390/ijms24119497

Figure Lengend Snippet: Oligonucleotide primer pairs used in this study.

Article Snippet: The resulting Vg4 plasmids were used as templates to generate ds Vg4 with the dsRNA synthesis kit (RNAsyn Biottech Co., Ltd., Jiangsu, Suzhou, China; ), following the manufacturer’s instructions: the preparation of dsRNA synthesis (DEPC water 51.6 μL, 5× transcription buffer 24 μL, NTP 20.4 μL, transcriptional template 6 μL, RNase enzyme inhibitor 3.6 μL, DTT 2.4 μL, RNA polymerase 12 μL), transcript at 37 °C for 1 h, add 12 μL DNaseI and react at 37 °C for 30 min, remove the template, and purify using NEB’s RNA purification kit (NEB, Ipswich, Massachusetts, USA) to obtain positive and negative Vg4 RNA; mix positive and negative Vg4 RNA in equal proportions and anneal to obtain ds Vg4 , with detailed synthesis methods provided in the .

Techniques: